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Subsequently, the GC cells were fixed (4% paraformaldehyde) for 5 minutes, permeated (0.1% Triton X-100) for 20 minutes, and blocked (5% bovine serum albumin (BSA)) for 1 hour

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However, concurrent hypermetabolism, hypercatabolism, and hypoanabolism provoked by systemic inflammation and catabolic factors, not reversed by conventional nutritional support, lead to progressive and irreversible impairment

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